Bacteriophage SP01 Gene Product 56 Inhibits Bacillus subtilis Cell Division by Interacting with FtsL and Disrupting Pbp2B and FtsW Recruitment
نویسندگان
چکیده
منابع مشابه
Analysis of the essential cell division gene ftsL of Bacillus subtilis by mutagenesis and heterologous complementation.
The ftsL gene is required for the initiation of cell division in a broad range of bacteria. Bacillus subtilis ftsL encodes a 13-kDa protein with a membrane-spanning domain near its N terminus. The external C-terminal domain has features of an alpha-helical leucine zipper, which is likely to be involved in the heterodimerization with another division protein, DivIC. To determine what residues ar...
متن کاملCooperative Recruitment of FtsW to the Division Site of Bacillus subtilis
Five essential proteins are known to assemble at the division site of Bacillus subtilis. However, the recruitment of the FtsW homolog is still unclear. Here, we take advantage of spore germination to facilitate the depletion of essential proteins and to study the divisome assembly in the absence of previous division events. We show that, unlike what has been shown for the Escherichia coli divis...
متن کاملTransduction in Bacillus subtilis by bacteriophage SPP1.
Lysates of the virulent bacteriophage SPP1 were shown to be capable of mediating generalized transduction. Suppressible mutants of this bacteriophage (sus) were capable of transduction at a lower multiplicity of infection than virulent SPP1. Linkage analysis demonstrated that bacteriophage SPP1 transduced segments of the genome equal in size to that transferred by SP10. This bacteriophage shoul...
متن کاملThe LysR-type transcriptional regulator YofA controls cell division through the regulation of expression of ftsW in Bacillus subtilis.
We have carried out a functional analysis of LysR family transcriptional regulators in Bacillus subtilis. The cell density of cultures of a yofA insertion mutant declined sharply after the end of exponential growth, as measured by optical density at 600 nm. Complementation in trans and analysis of isopropyl-beta-d-thiogalactopyranoside (IPTG)-dependent growth of an inducible yofA strain confirm...
متن کاملMultiple interactions between the transmembrane division proteins of Bacillus subtilis and the role of FtsL instability in divisome assembly.
About 11 essential proteins assemble into a ring structure at the surface of the cell to bring about cytokinesis in bacteria. Several of these proteins have their major domains located outside the membrane, forming an assembly that we call the outer ring (OR). Previous work on division in Bacillus subtilis has shown that four of the OR proteins-FtsL, DivIC, DivIB, and PBP 2B-are interdependent ...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 2020
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.00463-20